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The Decomposition of Aliphatic Esters by Aerobic Microorganisms W. D. LANGLEY, Engineering Research Associate W. B. DAVIS, Associate Professor and Head P. A. RICHARDS, Captain, USAF Environmental Engineering Division Civil Engineering Department Texas A&M University College Station, Texas INTRODUCTION Based on its ability to resolve and quantitatively analyze the various components of a multi-component system, gas chromatography is a valuable analytical technique for industrial wastewater characterization and treatment studies. Used in conjunction with the hydrogen flame ionization detector and with proper selection of operating variables the technique is capable of precise analysis of volatile organics in water solution at the part per million level without prior sample preparation. The technique and some of its applications to industrial wastes have been thoroughly described by Baker (1, 2). It has been shown to be of practical value in monitoring process waste streams and treatment plant operation at the Union Carbide plant in Charleston, West Virginia (3, 4). Various workers have applied the technique to the analysis of volatile fatty acids in digester sludge (5.6). In the authors' laboratories aqueous solution gas chromatography has been found to be an effective analytical method for the elucidation of multi-component substrate removal by aerobic microorganisms (7, 8, 9,10). Correlation of gas chromatography with Chemical Oxygen Demand removal (11) as well as with Biochemical Oxygen Demand (7) have shown the value of the method in interpreting the characteristics of the COD and BOD removal curves and identifying refractory compounds within the limitations of the technique. In addition, to providing the means whereby individual removal rates may be determined simultaneously, gas chromatography is also capable of detecting the appearance of volatile intermediate compounds in the biochemical decomposition or transformation of the primary substrates. These products may appear in the aqueous medium as the result of hydrolysis or oxidation. In some instances, short lived reduction products have been noted (12). The fate of these secondary substrates in the system may also be determined. In some of the early work with this method it was noted that n-butyl acetate underwent rapid hydrolysis in the laboratory activated sludge reactors. As a hydrolysis product 1-butanol appeared in the aqueous phase and its removal was followed subsequent to the disappearance of the ester. Inasmuch as the hydrolysis did not occur at neutral pH in the water solution alone, its occurrence in the microbial system was attributed to the presence of an esterase enzyme, the reaction proceeding according to the following equation. CH3COOC4H9 + H20 esterase»C4H9OH + CH3COOH - 229 -
Object Description
Purdue Identification Number | ETRIWC196820 |
Title | Decomposition of aliphatic esters by aerobic microorganisms |
Author |
Langley, W. D. Davis, W. B. (William B.) Richards, P. A. |
Date of Original | 1968 |
Conference Title | Proceedings of the 23rd Industrial Waste Conference |
Conference Front Matter (copy and paste) | http://earchives.lib.purdue.edu/u?/engext,15314 |
Extent of Original | p. 229-241 |
Series |
Engineering extension series no. 132 Engineering bulletin v. 53, no. 2 |
Collection Title | Engineering Technical Reports Collection, Purdue University |
Repository | Purdue University Libraries |
Rights Statement | Digital object copyright Purdue University. All rights reserved. |
Language | eng |
Type (DCMI) | text |
Format | JP2 |
Date Digitized | 2009-05-20 |
Capture Device | Fujitsu fi-5650C |
Capture Details | ScandAll 21 |
Resolution | 300 ppi |
Color Depth | 8 bit |
Description
Title | page 229 |
Collection Title | Engineering Technical Reports Collection, Purdue University |
Repository | Purdue University Libraries |
Rights Statement | Digital object copyright Purdue University. All rights reserved. |
Language | eng |
Type (DCMI) | text |
Format | JP2 |
Capture Device | Fujitsu fi-5650C |
Capture Details | ScandAll 21 |
Transcript | The Decomposition of Aliphatic Esters by Aerobic Microorganisms W. D. LANGLEY, Engineering Research Associate W. B. DAVIS, Associate Professor and Head P. A. RICHARDS, Captain, USAF Environmental Engineering Division Civil Engineering Department Texas A&M University College Station, Texas INTRODUCTION Based on its ability to resolve and quantitatively analyze the various components of a multi-component system, gas chromatography is a valuable analytical technique for industrial wastewater characterization and treatment studies. Used in conjunction with the hydrogen flame ionization detector and with proper selection of operating variables the technique is capable of precise analysis of volatile organics in water solution at the part per million level without prior sample preparation. The technique and some of its applications to industrial wastes have been thoroughly described by Baker (1, 2). It has been shown to be of practical value in monitoring process waste streams and treatment plant operation at the Union Carbide plant in Charleston, West Virginia (3, 4). Various workers have applied the technique to the analysis of volatile fatty acids in digester sludge (5.6). In the authors' laboratories aqueous solution gas chromatography has been found to be an effective analytical method for the elucidation of multi-component substrate removal by aerobic microorganisms (7, 8, 9,10). Correlation of gas chromatography with Chemical Oxygen Demand removal (11) as well as with Biochemical Oxygen Demand (7) have shown the value of the method in interpreting the characteristics of the COD and BOD removal curves and identifying refractory compounds within the limitations of the technique. In addition, to providing the means whereby individual removal rates may be determined simultaneously, gas chromatography is also capable of detecting the appearance of volatile intermediate compounds in the biochemical decomposition or transformation of the primary substrates. These products may appear in the aqueous medium as the result of hydrolysis or oxidation. In some instances, short lived reduction products have been noted (12). The fate of these secondary substrates in the system may also be determined. In some of the early work with this method it was noted that n-butyl acetate underwent rapid hydrolysis in the laboratory activated sludge reactors. As a hydrolysis product 1-butanol appeared in the aqueous phase and its removal was followed subsequent to the disappearance of the ester. Inasmuch as the hydrolysis did not occur at neutral pH in the water solution alone, its occurrence in the microbial system was attributed to the presence of an esterase enzyme, the reaction proceeding according to the following equation. CH3COOC4H9 + H20 esterase»C4H9OH + CH3COOH - 229 - |
Resolution | 300 ppi |
Color Depth | 8 bit |
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