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Substrate Interaction During Shock Loadings to Biological Treatment Processes K. K0M0LR1T, Research Assistant A. F. GAUDY, JR., Professor Bio-Engineering Laboratories School of Civil Engineering Oklahoma State University Stillwater, Oklahoma INTRODUCTION A considerable research effort concerning substrate interactions during waste water purification is currently under way in our laboratory. This line of investigation stems from the discovery of the occurrence of sequential substrate removal in heterogeneous populations (1). The finding of such an effect with heterogeneous populations is somewhat surprising and in many respects is in opposition to current concepts in the pollution control field, where it is usually considered that the many compounds in a waste are attacked concurrently by the many different species present in the waste. While this expectation does not appear unreasonable, we have amassed sufficient data to be able to state conclusively that sequential substrate removal in heterogeneous populations can occur (1,2, 3,4,5). Of greater significance to the pollution control field is the finding that a heterogeneous population which is already acclimated to a specific compound can be prevented from removing this compound by the action of a second substrate. This inhibition prevails until the second compound has been metabolized; thereafter the original compound is removed (2,3,4,5). It has been shown that the occurrence of this phenomenon is not restricted to growth systems, since we have found it to occur in large populations of pre-acclimated cells, which already possess a sufficient complement of the required enzyme(s) (2). We have also observed the phenomenon in nonproliferating systems (3). These findings have led us to the conclusion that there exists during cata- bolism a previously unstudied metabolic control mechanism, which acts to prevent the functioning of existing inducible enzyme(s), in addition to the well- known repression of the synthesis of inducible enzyme systems (3). Very recent investigations in the basic fields have shown that several mechanisms of metabolic control may be operative in bacteria. It would be beyond the scope of this report to discuss these in detail. However, since the various metabolic control mechanisms have serious ramifications to shock loading considerations and bear comparison with the new mechanism uncovered by our recent research, it is appropriate to review briefly the current state of knowledge in this area. In biosynthetic (anabolic) pathways two types of controls have been shown to prevent unnecessary expenditures of the energy and building blocks available to the cell (6, 7). The first of these, repression, operates to prevent the synthesis of enzymes involved in a biosynthetic pathway when the final product of those enzymes is supplied exogenously to the cell. This repression takes place at the level of gene function; that is, the copying of the structural information for enzyme - 796 -
Object Description
Purdue Identification Number | ETRIWC196462 |
Title | Substrate interaction during shock loadings to biological treatment processes |
Author |
Komolrit, K. Gaudy, Anthony F. |
Date of Original | 1964 |
Conference Title | Proceedings of the nineteenth Industrial Waste Conference |
Conference Front Matter (copy and paste) | http://earchives.lib.purdue.edu/u?/engext,11114 |
Extent of Original | p. 796-810 |
Series |
Engineering extension series no. 117 Engineering bulletin v. 49, no. 1(a)-2 |
Collection Title | Engineering Technical Reports Collection, Purdue University |
Repository | Purdue University Libraries |
Rights Statement | Digital object copyright Purdue University. All rights reserved. |
Language | eng |
Type (DCMI) | text |
Format | JP2 |
Date Digitized | 2009-05-19 |
Capture Device | Fujitsu fi-5650C |
Capture Details | ScandAll 21 |
Resolution | 300 ppi |
Color Depth | 8 bit |
Description
Title | page 796 |
Collection Title | Engineering Technical Reports Collection, Purdue University |
Repository | Purdue University Libraries |
Rights Statement | Digital object copyright Purdue University. All rights reserved. |
Language | eng |
Type (DCMI) | text |
Format | JP2 |
Capture Device | Fujitsu fi-5650C |
Capture Details | ScandAll 21 |
Transcript | Substrate Interaction During Shock Loadings to Biological Treatment Processes K. K0M0LR1T, Research Assistant A. F. GAUDY, JR., Professor Bio-Engineering Laboratories School of Civil Engineering Oklahoma State University Stillwater, Oklahoma INTRODUCTION A considerable research effort concerning substrate interactions during waste water purification is currently under way in our laboratory. This line of investigation stems from the discovery of the occurrence of sequential substrate removal in heterogeneous populations (1). The finding of such an effect with heterogeneous populations is somewhat surprising and in many respects is in opposition to current concepts in the pollution control field, where it is usually considered that the many compounds in a waste are attacked concurrently by the many different species present in the waste. While this expectation does not appear unreasonable, we have amassed sufficient data to be able to state conclusively that sequential substrate removal in heterogeneous populations can occur (1,2, 3,4,5). Of greater significance to the pollution control field is the finding that a heterogeneous population which is already acclimated to a specific compound can be prevented from removing this compound by the action of a second substrate. This inhibition prevails until the second compound has been metabolized; thereafter the original compound is removed (2,3,4,5). It has been shown that the occurrence of this phenomenon is not restricted to growth systems, since we have found it to occur in large populations of pre-acclimated cells, which already possess a sufficient complement of the required enzyme(s) (2). We have also observed the phenomenon in nonproliferating systems (3). These findings have led us to the conclusion that there exists during cata- bolism a previously unstudied metabolic control mechanism, which acts to prevent the functioning of existing inducible enzyme(s), in addition to the well- known repression of the synthesis of inducible enzyme systems (3). Very recent investigations in the basic fields have shown that several mechanisms of metabolic control may be operative in bacteria. It would be beyond the scope of this report to discuss these in detail. However, since the various metabolic control mechanisms have serious ramifications to shock loading considerations and bear comparison with the new mechanism uncovered by our recent research, it is appropriate to review briefly the current state of knowledge in this area. In biosynthetic (anabolic) pathways two types of controls have been shown to prevent unnecessary expenditures of the energy and building blocks available to the cell (6, 7). The first of these, repression, operates to prevent the synthesis of enzymes involved in a biosynthetic pathway when the final product of those enzymes is supplied exogenously to the cell. This repression takes place at the level of gene function; that is, the copying of the structural information for enzyme - 796 - |
Resolution | 300 ppi |
Color Depth | 8 bit |
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