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Kinetics of the Steady-State Bacterial Culture II. Variation in Synthesis L. J. HETLING, Public Health Service Fellow D. R. WASHINGTON, Associate Professor Department of Environmental Engineering Rensselaer Polytechnic Institute Troy, New York S. S. RAO, Research Microbiologist Canadian National Research Council Toronto, Canada INTRODUCTION One of the more important parameters used in the process design of activated sludge plants is the quantity ofsludge (cell material) produced or synthesized. The relationship between the quantity of cell material synthesized and the amount of substrate utilized is usually expressed as an effective yield coefficient (Y) having the units of weight of cell produced per weight of substrate removed. Experimentally, engineers and biologists have measured the effective yield coefficient by means of a material balance relating the accumulation of cell material to the removal of substrate under batch growth conditions. A tabulation of values found in this manner and reported in the biological and engineering literature are shown for pure culture in Table I and for mixed culture in Table II. These tables show a significant spread in the reported values of the effective yield coefficient, even for the same substrate, when measured by different investigations. It is the purpose of this paper to postulate reasons for these differences. VARIATIONS OF THE EFFECTIVE YIELD COEFFICIENT WITH SUBSTRATE AND ORGANISM The relationship between the effective yield coefficient and the nature of the organic substrate metabolized is an area in which there has been considerable speculation and confusion both in the biological and engineering literature. In the biological literature, most of the published work on this topic indicates that a stoichiometric relationship exists between the quantity of substrate oxidized and the quantity used in cell synthesis for a given organism and a given compound under similar conditions. Many attempts to relate the synthesis to the change in the free energy (AF) of the substrate were made but no experimental evidence could be found to support this relationship. In reviewing the subject, Lamanna and Mallette (13) conclude that the chemical nature of a substrate rather than its change in free energy controls synthesis during growth. A different approach to the problem was recently developed by Bauchop and Eldsen (14) who related observed cell growth to the calculated theoretical production of ATP from the energy source during metabolism. Experimental results - 687
Object Description
Purdue Identification Number | ETRIWC196455 |
Title | Kinetics of the steady-state bacterial culture. II. Variation in synthesis |
Author |
Hetling, Leo J., 1936- Washington, D. R. (Donald R.) Rao, S. S. |
Date of Original | 1964 |
Conference Title | Proceedings of the nineteenth Industrial Waste Conference |
Conference Front Matter (copy and paste) | http://earchives.lib.purdue.edu/u?/engext,11114 |
Extent of Original | p. 687-715 |
Series |
Engineering extension series no. 117 Engineering bulletin v. 49, no. 1(a)-2 |
Collection Title | Engineering Technical Reports Collection, Purdue University |
Repository | Purdue University Libraries |
Rights Statement | Digital object copyright Purdue University. All rights reserved. |
Language | eng |
Type (DCMI) | text |
Format | JP2 |
Date Digitized | 2009-05-19 |
Capture Device | Fujitsu fi-5650C |
Capture Details | ScandAll 21 |
Resolution | 300 ppi |
Color Depth | 8 bit |
Description
Title | page 687 |
Collection Title | Engineering Technical Reports Collection, Purdue University |
Repository | Purdue University Libraries |
Rights Statement | Digital object copyright Purdue University. All rights reserved. |
Language | eng |
Type (DCMI) | text |
Format | JP2 |
Capture Device | Fujitsu fi-5650C |
Capture Details | ScandAll 21 |
Transcript | Kinetics of the Steady-State Bacterial Culture II. Variation in Synthesis L. J. HETLING, Public Health Service Fellow D. R. WASHINGTON, Associate Professor Department of Environmental Engineering Rensselaer Polytechnic Institute Troy, New York S. S. RAO, Research Microbiologist Canadian National Research Council Toronto, Canada INTRODUCTION One of the more important parameters used in the process design of activated sludge plants is the quantity ofsludge (cell material) produced or synthesized. The relationship between the quantity of cell material synthesized and the amount of substrate utilized is usually expressed as an effective yield coefficient (Y) having the units of weight of cell produced per weight of substrate removed. Experimentally, engineers and biologists have measured the effective yield coefficient by means of a material balance relating the accumulation of cell material to the removal of substrate under batch growth conditions. A tabulation of values found in this manner and reported in the biological and engineering literature are shown for pure culture in Table I and for mixed culture in Table II. These tables show a significant spread in the reported values of the effective yield coefficient, even for the same substrate, when measured by different investigations. It is the purpose of this paper to postulate reasons for these differences. VARIATIONS OF THE EFFECTIVE YIELD COEFFICIENT WITH SUBSTRATE AND ORGANISM The relationship between the effective yield coefficient and the nature of the organic substrate metabolized is an area in which there has been considerable speculation and confusion both in the biological and engineering literature. In the biological literature, most of the published work on this topic indicates that a stoichiometric relationship exists between the quantity of substrate oxidized and the quantity used in cell synthesis for a given organism and a given compound under similar conditions. Many attempts to relate the synthesis to the change in the free energy (AF) of the substrate were made but no experimental evidence could be found to support this relationship. In reviewing the subject, Lamanna and Mallette (13) conclude that the chemical nature of a substrate rather than its change in free energy controls synthesis during growth. A different approach to the problem was recently developed by Bauchop and Eldsen (14) who related observed cell growth to the calculated theoretical production of ATP from the energy source during metabolism. Experimental results - 687 |
Resolution | 300 ppi |
Color Depth | 8 bit |
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