page 498 |
Previous | 1 of 17 | Next |
|
|
Loading content ...
A Procedure for Continuous Nitrifications Corrections During Warburg Respirometer Studies JAMES M. SYMONS, Research Public Health Engineer Engineering Section Division of Water Supply and Pollution Control Robert A. Taft Sanitary Engineering Center Cincinnati, Ohio ROGER LABONTE, Assistant Professor of Sanitary Engineering Ecole Polytechnique Montreal, Canada The Warburg Respirometer, a constant-volume manometer, permits the direct and continuous measurement of the oxygen utilization of an organic solution -- microorganism mixture in a closed system under conditions of constant temperature and agitation (1,2,3,4). One of the common uses of the Warburg Respirometer is for biodegradation studies. In general, these studies involve the mixing of a microorganism seed of specified origin with an organic solution in a Warburg Respirometer flask and measuring the rate and extent of oxygen uptake over a desired time period. Often, this oxygen uptake is compared with the oxygen uptake of a similar microorganism seed suspended in water. The rate of oxygen uptake, time lag to the beginning of metabolism, and the extent of total oxygen uptake of the fed sample, as compared to the control (unfed) sample, are used to evaluate the ease and extent of biodegradation of the organic matter under study (5,6,7,8). There are several problems associated with this technique; one of the major ones is the inability of the test to distinguish between oxygen taken up for the oxidation of organic matter to carbon dioxide and water and oxygen taken up for the oxidation of reduced nitrogen, i.e., nitrification. NITRIFICATION PROBLEM The nitrification reactions, as carried out by the autotrophic nitrifying bacteria, can be summarized by use of the following three equations. The reacting weights are given below each equation as well as oxygen taken up per unit of nitrogen converted. NOs + 2H+ + H20 (1) + wu. , i_1/9 On •»- 14 as N 48 1 3.43 \l/~> . i /n /~i ». "«2 T ■"* * v2 .^- 14 as N 16 1 1.14 - 498 - N03 (2)
Object Description
Purdue Identification Number | ETRIWC196345 |
Title | Procedure for continuous nitrifications corrections during Warburg Respirometer studies |
Author |
Symons, James M. Labonte, Roger |
Date of Original | 1963 |
Conference Title | Proceedings of the eighteenth Industrial Waste Conference |
Conference Front Matter (copy and paste) | http://earchives.lib.purdue.edu/cdm4/document.php?CISOROOT=/engext&CISOPTR=10285&REC=1 |
Extent of Original | p. 498-514 |
Collection Title | Engineering Technical Reports Collection, Purdue University |
Repository | Purdue University Libraries |
Rights Statement | Digital object copyright Purdue University. All rights reserved. |
Language | eng |
Type (DCMI) | text |
Format | JP2 |
Date Digitized | 2009-05-18 |
Capture Device | Fujitsu fi-5650C |
Capture Details | ScandAll 21 |
Resolution | 300 ppi |
Color Depth | 8 bit |
Description
Title | page 498 |
Collection Title | Engineering Technical Reports Collection, Purdue University |
Repository | Purdue University Libraries |
Rights Statement | Digital object copyright Purdue University. All rights reserved. |
Language | eng |
Type (DCMI) | text |
Format | JP2 |
Capture Device | Fujitsu fi-5650C |
Capture Details | ScandAll 21 |
Transcript | A Procedure for Continuous Nitrifications Corrections During Warburg Respirometer Studies JAMES M. SYMONS, Research Public Health Engineer Engineering Section Division of Water Supply and Pollution Control Robert A. Taft Sanitary Engineering Center Cincinnati, Ohio ROGER LABONTE, Assistant Professor of Sanitary Engineering Ecole Polytechnique Montreal, Canada The Warburg Respirometer, a constant-volume manometer, permits the direct and continuous measurement of the oxygen utilization of an organic solution -- microorganism mixture in a closed system under conditions of constant temperature and agitation (1,2,3,4). One of the common uses of the Warburg Respirometer is for biodegradation studies. In general, these studies involve the mixing of a microorganism seed of specified origin with an organic solution in a Warburg Respirometer flask and measuring the rate and extent of oxygen uptake over a desired time period. Often, this oxygen uptake is compared with the oxygen uptake of a similar microorganism seed suspended in water. The rate of oxygen uptake, time lag to the beginning of metabolism, and the extent of total oxygen uptake of the fed sample, as compared to the control (unfed) sample, are used to evaluate the ease and extent of biodegradation of the organic matter under study (5,6,7,8). There are several problems associated with this technique; one of the major ones is the inability of the test to distinguish between oxygen taken up for the oxidation of organic matter to carbon dioxide and water and oxygen taken up for the oxidation of reduced nitrogen, i.e., nitrification. NITRIFICATION PROBLEM The nitrification reactions, as carried out by the autotrophic nitrifying bacteria, can be summarized by use of the following three equations. The reacting weights are given below each equation as well as oxygen taken up per unit of nitrogen converted. NOs + 2H+ + H20 (1) + wu. , i_1/9 On •»- 14 as N 48 1 3.43 \l/~> . i /n /~i ». "«2 T ■"* * v2 .^- 14 as N 16 1 1.14 - 498 - N03 (2) |
Resolution | 300 ppi |
Color Depth | 8 bit |
Tags
Comments
Post a Comment for page 498