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Purdue University Veterinary Medical VOLUME 10, NUMBER 2 SUMMER- FALL 1984 Neuropharmacology at Purdue Significance of Dopamine Sulfate in Mammals Investigated of dopamine sulfate in the blood is complicated by the finding of graduate student, Grace Kao, D.V.M., that the drug (probenecid) commonly used to block transport for studies of this type also in- Dopamine is an endogenous catecholamine which functions as a neurotransmitter and neurohormone. Dopamine sulfate is the predominant form of dopamine which occurs in the blood of most higher mammals. Our laboratory is currently investigating the sources, functions, and metabolic activity of dopamine sulfate in mammals. We have recently developed several novel methods for measurement of dopamine sulfate isomers in biological samples. The most sensitive technique uses high performance liquid chromatography (HPLC) coupled with dual electrode electrochemical detection and can detect as little as 10"10 grams of either dopamine sulfate isomer (see figure). Patrick Swann, a graduate student, has developed a sample clean-up procedure which allows us to measure trace amounts of dopamine sulfate in a variety of "dirty" biological samples, such as blood and urine, and now, along with fellow student, Mary Scott, is investigating the pharmacokinetic properties and metabolic conversions of dopamine sulfate in dogs. The sources of the substantial amounts of dopamine sulfate in the blood are also being studied. Linda Toth, Ph.D., a veterinary medical student and part-time postdoctoral fellow, has demonstrated, in vitro, that platelets from human blood accumulate free dopamine and convert up CH2-CHj- Dr. Mary Ann Elchisak (left) and graduate students, Mary Scott and Patrick Swann, conduct experiments to measure dopamine sulfate. HO' DA - 3 - O- sulfate *T?r CH2-CH2-NH2 CH2-CH2-NH2 / w,?+t.oov a^^/C^ w2saoov -► I ,CH2_CH2-NH2 nnPAMINF OA - 4 - O - sulfate Proposed mechanism for dual-electrode detection of dopamine (DA) sulfate. As each isomer elutes from the HPLC system (not shown), it is oxided to a quinone at the upstream (Wl) electrode. The quinone is reduced to free DA at the downstream (W2) electrode by "recapture " of electrons. The electrical response at W2 is measured and used to quantitate the amount of DA sulfate in a sample. The distinction between the two DA sulfate isomers is maintained as a temporal separation in the HPLC procedure. to 60 percent of it to dopamine sulfate. Experiments conducted in the isolated ^effused rat kidncv/ bv< resenrch associate Moafak Jasim, D.V.M., in collaboration with Philip Mayer, Ph.D., in the School of Pharmacy, indicate that the rat kidney also converts free dopamine to dopamine sulfate. These studies suggest that both platelets and kidney are likely sources for much of the dopamine sulfate found in the blood. Study of the brain as a source hibits the enzyme which converts dopamine to dopamine sulfate. Results of these studies are increasing our understanding of catecholamine synthesis and metabolic mechanisms. Ultimately, they might aid in the treatment of diseases, such as essential hypertension and various neurological disorders, in which catecholamines are thought to be contributing factors. Mary Ann Elchisak, Ph.D. Silver Anniversary Development Campaign Yeager & Sullivan Contribute 1st Check Charles Yeager and his daughter, Dr. Marianne Ash of Yeager & Sullivan, Flora, Indiana, presented a check for $10,000 to Dean Jack J. Stockton in June 1984. It was the first contribution received for the SVM Silver Anniversary Development Campaign. The V'eager[Sullivan pledge of $50,000 over the five years of the campaign also makes them the first member of our School of Veterinary Medicine Leadership Club. The interest and generosity of the Yeager family is greatly appreciated by the School of Veterinary Medicine. Yeager & Sullivan is a poultry, swine and feed business. Marianne Yeager Ash is a DVM '77 graduate. Her husband, Stephen P. Ash, M.D., is an adjunct associate professor in the Department of Small Animal Clinics. ^r1 Wayne Woodrow Kirkham 1918-1984 Retiring from Purdue University on 30 June 1984, as Professor Emeritus and moving to Panama City, Florida, in mid- July, Wayne celebrated his 66th birthday there on 20 July in the midst of the family which he loved so deeply. On 24 July, without signals of serious problems, death came while he slept. Prior to earning degrees from Texas A&M University (DVM'41 and MS'55) and from Iowa State University (Ph.D. Microbiology'62), he practiced and served with the USDA in his native Hoosier state. He spent a year in 1955-56 on the staff at Kansas State University. Following graduate work, he spent six years in Florida with the Florida Department of Agriculture at Kissimmee and with the University of Florida at Gainesville, coming to Purdue in July 1968, to head the Animal Disease Diagnostic Laboratory. Many improvements were effected in this unit, which he headed until July 1978, when he assumed the title of veterinary diagnostician. People and horses were his great loves. Upon learning of his death, two former students wrote: "... It was a shock to learn of the sudden death of Dr. Kirkham, our good friend and mentor. The friendship and help he gave us both during veterinary school and afterwards as we initiated our careers has been deeply appreciated and is an example to us. His generosity cannot be repaid except as we try to live up to his example." An eloquent testimony to a warm, interested, concerned, and contributing colleague. The family asked that those wishing to remember Wayne may contribute to the IVMA Scholarship Fund in his memory. ^i^il
Object Description
Title | Purdue University veterinary medical update, 1984, v. 10, no. 2 (Summer-Fall) |
Subjects (MeSH) |
Veterinary Medicine Education, Veterinary |
Creators | Purdue University. School of Veterinary Medicine |
Purdue Identification Number | PSVM00102 |
Subjects (LCSH) | Veterinary medicine--Study and teaching (Higher) |
Genre | Periodical |
Coverage | United States |
Date of Original | 1984 |
Type | text |
Format | JP2 |
Collection Title | SVM Report |
Repository | Purdue University Libraries |
Language | eng |
Rights Statement | Digital object copyright Purdue University. All rights reserved. |
Date Digitized | 2009-08-06 |
Digitization Information | Original scanned at 300 ppi on a Bookeye 3 scanner using Bookeye 3 internal software, with 24 bit color depth. Display images generated in CONTENTdm as JP2000s; file format for archival copy is uncompressed TIF format. |
URI | ark:/34231/c63r0qs0 |
Description
Title | page 1 |
Subjects (MeSH) |
Veterinary Medicine Education, Veterinary |
Creators | Purdue University. School of Veterinary Medicine |
Subjects (LCSH) | Veterinary medicine--Study and teaching (Higher) |
Genre | Periodical |
Coverage | United States |
Type | text |
Format | JP2 |
Collection Title | SVM Report |
Repository | Purdue University Libraries |
Language | eng |
Rights Statement | Digital object copyright Purdue University. All rights reserved. |
Digitization Information | Original scanned at 300 ppi on a Bookeye 3 scanner using Bookeye 3 internal software, with 24 bit color depth. Display images generated in CONTENTdm as JP2000s; file format for archival copy is uncompressed TIF format. |
Transcript | Purdue University Veterinary Medical VOLUME 10, NUMBER 2 SUMMER- FALL 1984 Neuropharmacology at Purdue Significance of Dopamine Sulfate in Mammals Investigated of dopamine sulfate in the blood is complicated by the finding of graduate student, Grace Kao, D.V.M., that the drug (probenecid) commonly used to block transport for studies of this type also in- Dopamine is an endogenous catecholamine which functions as a neurotransmitter and neurohormone. Dopamine sulfate is the predominant form of dopamine which occurs in the blood of most higher mammals. Our laboratory is currently investigating the sources, functions, and metabolic activity of dopamine sulfate in mammals. We have recently developed several novel methods for measurement of dopamine sulfate isomers in biological samples. The most sensitive technique uses high performance liquid chromatography (HPLC) coupled with dual electrode electrochemical detection and can detect as little as 10"10 grams of either dopamine sulfate isomer (see figure). Patrick Swann, a graduate student, has developed a sample clean-up procedure which allows us to measure trace amounts of dopamine sulfate in a variety of "dirty" biological samples, such as blood and urine, and now, along with fellow student, Mary Scott, is investigating the pharmacokinetic properties and metabolic conversions of dopamine sulfate in dogs. The sources of the substantial amounts of dopamine sulfate in the blood are also being studied. Linda Toth, Ph.D., a veterinary medical student and part-time postdoctoral fellow, has demonstrated, in vitro, that platelets from human blood accumulate free dopamine and convert up CH2-CHj- Dr. Mary Ann Elchisak (left) and graduate students, Mary Scott and Patrick Swann, conduct experiments to measure dopamine sulfate. HO' DA - 3 - O- sulfate *T?r CH2-CH2-NH2 CH2-CH2-NH2 / w,?+t.oov a^^/C^ w2saoov -► I ,CH2_CH2-NH2 nnPAMINF OA - 4 - O - sulfate Proposed mechanism for dual-electrode detection of dopamine (DA) sulfate. As each isomer elutes from the HPLC system (not shown), it is oxided to a quinone at the upstream (Wl) electrode. The quinone is reduced to free DA at the downstream (W2) electrode by "recapture " of electrons. The electrical response at W2 is measured and used to quantitate the amount of DA sulfate in a sample. The distinction between the two DA sulfate isomers is maintained as a temporal separation in the HPLC procedure. to 60 percent of it to dopamine sulfate. Experiments conducted in the isolated ^effused rat kidncv/ bv< resenrch associate Moafak Jasim, D.V.M., in collaboration with Philip Mayer, Ph.D., in the School of Pharmacy, indicate that the rat kidney also converts free dopamine to dopamine sulfate. These studies suggest that both platelets and kidney are likely sources for much of the dopamine sulfate found in the blood. Study of the brain as a source hibits the enzyme which converts dopamine to dopamine sulfate. Results of these studies are increasing our understanding of catecholamine synthesis and metabolic mechanisms. Ultimately, they might aid in the treatment of diseases, such as essential hypertension and various neurological disorders, in which catecholamines are thought to be contributing factors. Mary Ann Elchisak, Ph.D. Silver Anniversary Development Campaign Yeager & Sullivan Contribute 1st Check Charles Yeager and his daughter, Dr. Marianne Ash of Yeager & Sullivan, Flora, Indiana, presented a check for $10,000 to Dean Jack J. Stockton in June 1984. It was the first contribution received for the SVM Silver Anniversary Development Campaign. The V'eager[Sullivan pledge of $50,000 over the five years of the campaign also makes them the first member of our School of Veterinary Medicine Leadership Club. The interest and generosity of the Yeager family is greatly appreciated by the School of Veterinary Medicine. Yeager & Sullivan is a poultry, swine and feed business. Marianne Yeager Ash is a DVM '77 graduate. Her husband, Stephen P. Ash, M.D., is an adjunct associate professor in the Department of Small Animal Clinics. ^r1 Wayne Woodrow Kirkham 1918-1984 Retiring from Purdue University on 30 June 1984, as Professor Emeritus and moving to Panama City, Florida, in mid- July, Wayne celebrated his 66th birthday there on 20 July in the midst of the family which he loved so deeply. On 24 July, without signals of serious problems, death came while he slept. Prior to earning degrees from Texas A&M University (DVM'41 and MS'55) and from Iowa State University (Ph.D. Microbiology'62), he practiced and served with the USDA in his native Hoosier state. He spent a year in 1955-56 on the staff at Kansas State University. Following graduate work, he spent six years in Florida with the Florida Department of Agriculture at Kissimmee and with the University of Florida at Gainesville, coming to Purdue in July 1968, to head the Animal Disease Diagnostic Laboratory. Many improvements were effected in this unit, which he headed until July 1978, when he assumed the title of veterinary diagnostician. People and horses were his great loves. Upon learning of his death, two former students wrote: "... It was a shock to learn of the sudden death of Dr. Kirkham, our good friend and mentor. The friendship and help he gave us both during veterinary school and afterwards as we initiated our careers has been deeply appreciated and is an example to us. His generosity cannot be repaid except as we try to live up to his example." An eloquent testimony to a warm, interested, concerned, and contributing colleague. The family asked that those wishing to remember Wayne may contribute to the IVMA Scholarship Fund in his memory. ^i^il |
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