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VOLUME 14, NUMBER 2 MARCH 1989 The Purdue Practitioner Sabbatical Program Participants Give the Program High Marks "I know I've seen this before. I didn't know what it was then either." "Doctor, you have two clients waiting." All too often the pressures of daily practice prevent veterinary practitioners from pursuing questions about specific disease or developing new techniques. Many times in solo practices, or even multi-person practices, schedules are such that other colleagues are not available for consultation. The Purdue Practitioner Sabbatical Program provides a unique opportunity for the practicing veterinarian to get away and work on specific problems. The program is very simple. Each sabbatical person needs to identify a faculty mentor. If the person wanted to work on surgery, someone in the Surgery Section would be identified. If the interest was in improving radiographic interpretation skills or perhaps developing skills in diagnostic ultrasound, someone in the Imaging Section would be identified. The hallmark of this program is flexibility. Normally, the practitioner registers for the program one week at a time. A week is defined as five working days. However, the days can be scheduled in any manner suitable to each party. In the Imaging Section, it has been popular to schedule one day every other week. Veterinarians within the state have used a day off during the week for their sabbatical time and commuted to Purdue. This allows minimal time away from the practice and fits well into the Purdue schedule. Dr. Dick Goebel (PUR'68), director of the Veterinary Teaching Hospital, gives the program a strong endorsement. "We want the teaching hospital to be a good model for our students. The practitioners who return for sabbaticals are excellent role models; their presence says to our students in yet another way that their education is a lifelong process." Dr. Cliff Blakemore, Purdue faculty member since 1961, has this to say about the Practitioner Sabbatical Program: "This program provides a unique opportunity for continuing education in a stimulating and very special environment. It combines the pleasure of a casual visit with intensive continuing education. The student has free access to all of our learning resources (clinical cases, rounds, impromptu discussions, audiovisuals, library, computer lab, all-School seminars, outside speakers, etc.) with the friendly guidance of a mentor to help make the most of the opportunities available. As our profession grows and changes, we welcome this opportunity to involve working professionals in our daily activities. I expect this kind of interaction to become a standard feature of clinical education." Dr. Ron Doversberger (PUR'75), Mishawaka, IN: "My experience during my one week in small animal surgery was a positive one. Simply sharing the time with students and faculty strengthened the bond and pride that I feel as a Purdue graduate. The greatest practical value... exposure to new suture materials and wound closure techniques, methods of menostasis, and In March 1985 Dr. Bruce Sharp's untimely death was a profound loss to his family, colleagues, and friends. The Calumet Area Veterinary Medical Association (District I) initiated an endowment memorial fund as an expression of their respect and affection for Bruce. Through the contributions of his friends from around the state and country, the principle has grown to almost $20,000. Interest is to be used to assist DVMs in participating in the Practitioner's Sabbatical Program at Purdue SVM. Funds can be used to defray any of the costs associated with the program, i.e., tuition, fees, transportation, housing, cost of leaving practice, etc. Most participants in the sabbatical program have declined to apply for the scholarship. In order to fulfill its purpose as a living memorial to Bruce Sharp, the scholarships need to be used. Practitioners applying for the Sabbatical Program should ask SVM Continuing Education Office for the simple one-page application form. bandaging methods ... sharing in the diagnostic work-up, i.e., the physical exam, radiographic and ultrasound evaluations." Dr. Rod Duncan (PUR'75), Indianapolis, IN: "By using one day at a time, I was able to absorb a tremendous amount of information and then return to my practice and digest, assimilate, apply the information ... I intend to do a practitioner sabbatical annually and make it a key part of my continuing education." Dr. Dick Headley (PUR'67), Mishawaka, IN: "I have experienced two separate sessions... I expect to apply for another late next year. The ability to ask a question and be able to expand upon it in a one-on-one situation is invaluable ... the time spent in rounds was invaluable ... I feel this experience has made me a better practitioner." Dr. Bill Blevins (PUR'68), faculty member, West Lafayette, IN: "This is a situation where everyone wins ... the sabbatical program has provided benefit for Purdue and its faculty as well as for the participants in the program. The $200 per week fee is placed into an account for the mentor to use as discretionary funds. The interaction between the sabbatical person and the mentor helps provide perspective and relevance for the faculty person. Relevance to the DVM program is enhanced by interactions between the sabbatical veterinarian and DVM students." Any veterinarian interested in taking advantage of this opportunity should call (317)494-1107,tocontactapotentialsmall animal mentor, (317) 494-8548, to contact a large animal mentor; or (317) 494-7617, Continuing Education Office, for additional information. „ „. , , n , . Dr. Richard Goebel Flow Cytometry Facility at Purdue HUMAN LEUKOCYTES New Research Capabilities Established in SVM An important area that is targeted for development by the Cancer Center and Purdue University is the improvement of cell biology resources on campus. Until the technology of laser-based flow cytometry became widely available, cell biologists and immunologists had been hampered by their inability to quantitatively and qualitatively analyze a specific subpopulation of cells within a heterogenous population. Their approaches had been limited to descriptive (microscopic slides and stains) or chemical (biophysical and enzymologic reactions) studies, both very labor intensive and neither very satisfactory. Furthermore, the isolation of such subpopulations for additional studies with any degree of purity has proven difficult, if not impossible. These frustrations were amply felt by cancer researchers on the Purdue campus and motivated them to seek the establish- mentofacentral flow cytometry facility. A group of investigators, which included Drs. Ashendel, Chan, Crane, Geahlen, Morre, and Pfeifer submitted a Shared Instrumentation Grant Application to the NIH requesting funds for the purchase of flow cytometry equipment. The application was w funded, and the following instruments were purchased from EPICS division of Coulter Electronics: - one EPICS 753 dual argon laser flow cytometer/cell sorter; - one EPICS C single argon laser flow cytometer/cell sorter; - one EASY 88 off line data analysis computing system. The School of Veterinary Medicine renovated the laboratory space required to house the equipment temporarily, with the intention of moving the instruments into the flow cytometry suite within the Life Sciences Research Building when the space becomes available for occupancy in 1989. The flow cytometry facility was under the direction of Dr. Thomas Chan, Department of Veterinary Physiology and Pharmacology, during the initial set up phase with the assistance of a dedicated operator, Linda Sands, from the Department of Pathobiology. After the instruments were installed it became clear to the facility committee that it was necessary to recruit expertise in the area of flow cytometry. The Department of Veterinary Physiology and Pharmacology has recently recruited Dr. J. Paul Robinson, a nationally recognized flow cytomctrist from the University of Michigan, to fill an immunopharmacologist position in the department and to direct the flow cy tometcr laboratory. Dr. Robinson began his appointment at Purdue July 1, 1988, and has been meeting with different research groups on campus to discuss flow cytometry as an investigative tool in biology and medicine. Dr. Robinson will continue to be aided by Linda Sands and by technician, Cathy Noble. Also assisting is an expert in computer monitoring and data analysis, Stephen Kelly. What is Flow Cytometry? Flow cytometry is a technology which uses a laser beam to interrogate cells as they pass through the beam. The fascinating feature about this is that the cells are not damaged in any way by the laser. This is primarily because the cells pass through the beam in about 1/10,000 of one second and also because the cells are surrounded by a sheath of fluid. As the cells pass through the beam, a number of measurements are made Forward Light Scatter Figure 1. by detectors nearby. Since light is scattered in many directions by the cells breaking the laser beam, we can measure a number of properties of the cells by making a multiparameter analysis of these signals. The most common property we can measure is emitted fluorescence of fluoro- chromes attached to the cell. These could be via attached antibodies or by fluorescent dyes which are absorbed into the cell organelles by one means or other. Differences in cells can be characterized by the way they interact with these fluorochromes. Simi- continued on page 2
Object Description
Title | Purdue University veterinary medical update, 1989, v. 14, no. 2 (March) |
Subjects (MeSH) |
Veterinary Medicine Education, Veterinary |
Creators | Purdue University. School of Veterinary Medicine |
Purdue Identification Number | PSVM00142 |
Subjects (LCSH) | Veterinary medicine--Study and teaching (Higher) |
Genre | Periodical |
Coverage | United States |
Date of Original | 1989 |
Type | text |
Format | JP2 |
Collection Title | SVM Report |
Repository | Purdue University Libraries |
Language | eng |
Rights Statement | Digital object copyright Purdue University. All rights reserved. |
Date Digitized | 2009-08-06 |
Digitization Information | Original scanned at 300 ppi on a Bookeye 3 scanner using Bookeye 3 internal software, with 24 bit color depth. Display images generated in CONTENTdm as JP2000s; file format for archival copy is uncompressed TIF format. |
URI | ark:/34231/c62r3pnm |
Description
Title | page 1 |
Subjects (MeSH) |
Veterinary Medicine Education, Veterinary |
Creators | Purdue University. School of Veterinary Medicine |
Subjects (LCSH) | Veterinary medicine--Study and teaching (Higher) |
Genre | Periodical |
Coverage | United States |
Type | text |
Format | JP2 |
Collection Title | SVM Report |
Repository | Purdue University Libraries |
Language | eng |
Rights Statement | Digital object copyright Purdue University. All rights reserved. |
Digitization Information | Original scanned at 300 ppi on a Bookeye 3 scanner using Bookeye 3 internal software, with 24 bit color depth. Display images generated in CONTENTdm as JP2000s; file format for archival copy is uncompressed TIF format. |
Transcript | VOLUME 14, NUMBER 2 MARCH 1989 The Purdue Practitioner Sabbatical Program Participants Give the Program High Marks "I know I've seen this before. I didn't know what it was then either." "Doctor, you have two clients waiting." All too often the pressures of daily practice prevent veterinary practitioners from pursuing questions about specific disease or developing new techniques. Many times in solo practices, or even multi-person practices, schedules are such that other colleagues are not available for consultation. The Purdue Practitioner Sabbatical Program provides a unique opportunity for the practicing veterinarian to get away and work on specific problems. The program is very simple. Each sabbatical person needs to identify a faculty mentor. If the person wanted to work on surgery, someone in the Surgery Section would be identified. If the interest was in improving radiographic interpretation skills or perhaps developing skills in diagnostic ultrasound, someone in the Imaging Section would be identified. The hallmark of this program is flexibility. Normally, the practitioner registers for the program one week at a time. A week is defined as five working days. However, the days can be scheduled in any manner suitable to each party. In the Imaging Section, it has been popular to schedule one day every other week. Veterinarians within the state have used a day off during the week for their sabbatical time and commuted to Purdue. This allows minimal time away from the practice and fits well into the Purdue schedule. Dr. Dick Goebel (PUR'68), director of the Veterinary Teaching Hospital, gives the program a strong endorsement. "We want the teaching hospital to be a good model for our students. The practitioners who return for sabbaticals are excellent role models; their presence says to our students in yet another way that their education is a lifelong process." Dr. Cliff Blakemore, Purdue faculty member since 1961, has this to say about the Practitioner Sabbatical Program: "This program provides a unique opportunity for continuing education in a stimulating and very special environment. It combines the pleasure of a casual visit with intensive continuing education. The student has free access to all of our learning resources (clinical cases, rounds, impromptu discussions, audiovisuals, library, computer lab, all-School seminars, outside speakers, etc.) with the friendly guidance of a mentor to help make the most of the opportunities available. As our profession grows and changes, we welcome this opportunity to involve working professionals in our daily activities. I expect this kind of interaction to become a standard feature of clinical education." Dr. Ron Doversberger (PUR'75), Mishawaka, IN: "My experience during my one week in small animal surgery was a positive one. Simply sharing the time with students and faculty strengthened the bond and pride that I feel as a Purdue graduate. The greatest practical value... exposure to new suture materials and wound closure techniques, methods of menostasis, and In March 1985 Dr. Bruce Sharp's untimely death was a profound loss to his family, colleagues, and friends. The Calumet Area Veterinary Medical Association (District I) initiated an endowment memorial fund as an expression of their respect and affection for Bruce. Through the contributions of his friends from around the state and country, the principle has grown to almost $20,000. Interest is to be used to assist DVMs in participating in the Practitioner's Sabbatical Program at Purdue SVM. Funds can be used to defray any of the costs associated with the program, i.e., tuition, fees, transportation, housing, cost of leaving practice, etc. Most participants in the sabbatical program have declined to apply for the scholarship. In order to fulfill its purpose as a living memorial to Bruce Sharp, the scholarships need to be used. Practitioners applying for the Sabbatical Program should ask SVM Continuing Education Office for the simple one-page application form. bandaging methods ... sharing in the diagnostic work-up, i.e., the physical exam, radiographic and ultrasound evaluations." Dr. Rod Duncan (PUR'75), Indianapolis, IN: "By using one day at a time, I was able to absorb a tremendous amount of information and then return to my practice and digest, assimilate, apply the information ... I intend to do a practitioner sabbatical annually and make it a key part of my continuing education." Dr. Dick Headley (PUR'67), Mishawaka, IN: "I have experienced two separate sessions... I expect to apply for another late next year. The ability to ask a question and be able to expand upon it in a one-on-one situation is invaluable ... the time spent in rounds was invaluable ... I feel this experience has made me a better practitioner." Dr. Bill Blevins (PUR'68), faculty member, West Lafayette, IN: "This is a situation where everyone wins ... the sabbatical program has provided benefit for Purdue and its faculty as well as for the participants in the program. The $200 per week fee is placed into an account for the mentor to use as discretionary funds. The interaction between the sabbatical person and the mentor helps provide perspective and relevance for the faculty person. Relevance to the DVM program is enhanced by interactions between the sabbatical veterinarian and DVM students." Any veterinarian interested in taking advantage of this opportunity should call (317)494-1107,tocontactapotentialsmall animal mentor, (317) 494-8548, to contact a large animal mentor; or (317) 494-7617, Continuing Education Office, for additional information. „ „. , , n , . Dr. Richard Goebel Flow Cytometry Facility at Purdue HUMAN LEUKOCYTES New Research Capabilities Established in SVM An important area that is targeted for development by the Cancer Center and Purdue University is the improvement of cell biology resources on campus. Until the technology of laser-based flow cytometry became widely available, cell biologists and immunologists had been hampered by their inability to quantitatively and qualitatively analyze a specific subpopulation of cells within a heterogenous population. Their approaches had been limited to descriptive (microscopic slides and stains) or chemical (biophysical and enzymologic reactions) studies, both very labor intensive and neither very satisfactory. Furthermore, the isolation of such subpopulations for additional studies with any degree of purity has proven difficult, if not impossible. These frustrations were amply felt by cancer researchers on the Purdue campus and motivated them to seek the establish- mentofacentral flow cytometry facility. A group of investigators, which included Drs. Ashendel, Chan, Crane, Geahlen, Morre, and Pfeifer submitted a Shared Instrumentation Grant Application to the NIH requesting funds for the purchase of flow cytometry equipment. The application was w funded, and the following instruments were purchased from EPICS division of Coulter Electronics: - one EPICS 753 dual argon laser flow cytometer/cell sorter; - one EPICS C single argon laser flow cytometer/cell sorter; - one EASY 88 off line data analysis computing system. The School of Veterinary Medicine renovated the laboratory space required to house the equipment temporarily, with the intention of moving the instruments into the flow cytometry suite within the Life Sciences Research Building when the space becomes available for occupancy in 1989. The flow cytometry facility was under the direction of Dr. Thomas Chan, Department of Veterinary Physiology and Pharmacology, during the initial set up phase with the assistance of a dedicated operator, Linda Sands, from the Department of Pathobiology. After the instruments were installed it became clear to the facility committee that it was necessary to recruit expertise in the area of flow cytometry. The Department of Veterinary Physiology and Pharmacology has recently recruited Dr. J. Paul Robinson, a nationally recognized flow cytomctrist from the University of Michigan, to fill an immunopharmacologist position in the department and to direct the flow cy tometcr laboratory. Dr. Robinson began his appointment at Purdue July 1, 1988, and has been meeting with different research groups on campus to discuss flow cytometry as an investigative tool in biology and medicine. Dr. Robinson will continue to be aided by Linda Sands and by technician, Cathy Noble. Also assisting is an expert in computer monitoring and data analysis, Stephen Kelly. What is Flow Cytometry? Flow cytometry is a technology which uses a laser beam to interrogate cells as they pass through the beam. The fascinating feature about this is that the cells are not damaged in any way by the laser. This is primarily because the cells pass through the beam in about 1/10,000 of one second and also because the cells are surrounded by a sheath of fluid. As the cells pass through the beam, a number of measurements are made Forward Light Scatter Figure 1. by detectors nearby. Since light is scattered in many directions by the cells breaking the laser beam, we can measure a number of properties of the cells by making a multiparameter analysis of these signals. The most common property we can measure is emitted fluorescence of fluoro- chromes attached to the cell. These could be via attached antibodies or by fluorescent dyes which are absorbed into the cell organelles by one means or other. Differences in cells can be characterized by the way they interact with these fluorochromes. Simi- continued on page 2 |
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